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Immunological Investigations
A Journal of Molecular and Cellular Immunology
Volume 20, 1991 - Issue 7
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Original Article

Standardization of Elisa for the Detection of Anti-Cardiolipin Antibodies - Effect of Non-Specific IgG Binding

, , , &
Pages 583-593 | Published online: 07 Jul 2009
 

Abstract

Various frequencies of anti-cardiolipin antibodies reported in patients with systemic lupus erythematosus and other autoimmune and infectious diseases necessitates the need for standardization of the immunoassay. We report here the usefulness of “no-antigen” control for each serum in determining the actual value of the anti-phospholipid antibodies. This non-specific “no-antigen” binding was quite variable from serum to serum and in general was higher in the patient population than in healthy individuals which served as controls. This non-specific binding may be associated with the increased IgG content of the sera as shown by the linear association of the absorbance of the back-ground readings to increases in IgG concentration (correlation coefficient 0.98).

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