Synergistic Activation of Serum Amyloid a (SAA) by IL-6 and IL-1 in Combination on Human Hep 3B Hepatoma Cell Line. Role of PGE2 and IL-1 Receptor Antagonist

Abstract

Serum amyloid A (SAA) protein is a major acute phase reactant in human and many other species. Infections and traumatic inflammation are characterized by a rapid increase of SAA; its concentration in the plasma may augment many-fold. Cytokines such as IL-1 and IL-6 are considered mediators of acute phase protein synthesis. The most accredited mechanism of action of IL-1 in inflammatory diseases is the stimulation of PGE2 release, which is highly dependent on the concentration of IL-1. In this study we found that human Hep 3B hepatoma cells treated with the combination of hrIL-6 (10ng/ml) plus hrIL-1 (1ng/ml) produced an augmentation in steady-state levels of SAA mRNA (87%) compared to hrIL-6 (10ng/ml) plus PGE2 (5 μM), which induced an increase of only 33%, compared to IL-6 alone, while cells treated with hrIL-6 plus PGE2 (0.5 μM) had a similar effect as hrIL-6 did alone. Moreover, the addition of exogenous PGE2 (5 μM) to the cell cultures produced no significant increase in concentration of SAA mRNA compared to the control. In addition, according to the data obtained by the blot analysis we also found, by ELISA method, that hrIL-6 acts in synergism with hrIL-1 on SAA protein secretion in human Hep 3B hepatoma cell cultures after 48 h incubation. In fact, the cell cultures treated with hrIL-6 plus hrIL-1 caused a higher release approximately 1.5–4-fold of SAA protein than the cells treated with IL-6 plus PGE2 5μM or IL-1 + PGE2 5μM, respectively. The synergistic effect of hrIL-6 plus hrIL-1ß was inhibited by hrIL-1 receptor antagonist (hrIL-1ra) 50 μg/ml, a protein which specifically binds to the IL-1 receptor and is structurally similar to IL-1ß but with no IL-1-like activity; while indomethacin (5 μM) was ineffective. These results strongly suggest that the synergism between hrIL-6 plus hrIL-1 on the transcription and the protein release of SAA release is not due to a PGE2-dependent process in human Hep 3B hepatoma cells. This finding highlights a specific biological effect of IL-1 not in relation to PGE2, suggesting a specific mechanism of action for IL-1 in regulating acute phase protein synthesis.