Analysis of clonal diversity in mouse immunoglobulin heavy chain genes selected for size of the antigen combining site

Abstract

Size-diversity of lg and T cell receptor antigen binding (CDR3) regions can be visualized by “CDR3 fingerprinting”, and provides an estimate of B- or T-cell repertoire complexity. The method does not identify clonal diversity, however, which can only be determined by random sequencing of the CDR3s. In this study we demonstrate that a combination of fingerprinting and single strand conformation polymorphism (SSCP) analysis can be used for a rapid estimatation of clonal diversity within mouse lg antigen binding regions selected for size. This application may be useful in the analysis of clonal expansion within B- and T-cell repertoires.