Dendritic Cells in Human Blood and Synovial Exudates

Abstract

Dendritic cells from human blood and synovial exudates are distinct from other leukocytes and are homogeneous by several criteria. Morphologically, their most prominent feature is numerous veils. Phenotypically, dendritic cells lack the surface antigens that identify monocytes, T cells, B cells, and NK cells. Human dendritic cells strongly express class I and class II MHC products, and have a distinct array of integrin and adhesin molecules. In many systems, dendritic cells are potent stimulators of T cell function. In the allogeneic mixed leukocyte reaction, for example, dendritic cells are 30–100 times more efficient than other cells in presenting transplantation antigens, for the induction of DNA synthesis, cytokine release, and generation of cytotoxic T cells. In addition, dendritic cells can induce the long-term clonal growth of T lymphocytes. Although dendritic cells are a minor subpopulation in human blood, new isolation protocols are available that permit efficient isolation and enrichment to>90%.

Key Words:

• dendritic cells
• human blood
• synovial exudates
• antigen presentation
• mixed leukocyte reaction