Effect of Ligustrazine on rat peritoneal mesothelial cells treated with lipopolysaccharide

Abstract

The apoptosis of peritoneal mesothelial cells (PMCs) and peritoneal fibrosis may induce failure of peritoneal membrane function. The study explored the changes of apoptosis and fibrosis in PMCs under lipopolysaccharides (LPS) culture and investigated whether Ligustrazine can affect LPS-induced apoptosis and fibrosis. We found that exposure of rat PMCs to 5 mg·L⁻¹ LPS for 24 h resulted in a significant induction of apoptosis and increased levels in Reactive oxygen species, and caspase-3 activity. Fibronectin, Collagen I, p-p38, and matrix metalloprotein-9 (MMP-9) levels were also significantly increased by LPS. But superoxide dismutase levels were remarkably decreased. Ligustrazine can restore the changes induced by LPS. The protective effect of Ligustrazine on LPS-induced apoptosis and fibrosis may act through inhibition of oxidative stress and p38/MAPKS, ROS/MMP-9 activation in PMCs.

Keywords:

• Ligustrazine
• MMP-9
• oxidative stress
• p38/MAPKS
• RPMCs

Disclosure statement

The authors reported no proprietary or commercial interest in any product mentioned or concept discussed in the article. The authors have no conflicts of interest to declare.

Funding information

This work was supported by highly educated people funded projects of Xinxiang Medical University.