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Research Article

Regulation of Th-POK and Runx3 in T cell development in human thymoma

, , , , , & show all
Pages 653-660 | Received 08 Jan 2009, Accepted 03 Jun 2009, Published online: 01 Oct 2009
 

Abstract

Thymoma is a thymic epithelial neoplasm which induces T cell development. However, the frequency of mature CD4+ T cells in thymomas is lower than in normal thymi. Recently, CD4/CD8 lineage commitment has been elucidated in animal model. The zinc finger transcription factor Th-POK is a critical factor to CD4+ T cell development in CD4/CD8 lineage commitment, whereas CD8+ T cell development requires the transcription factor Runx3. These factors antagonize in CD4/CD8 lineage commitment. In this study, we examined Th-POK and Runx3 mRNA expression in the T cell subsets of human normal thymus and thymoma. A quantitative reverse transcriptase-polymerase chain reaction examination revealed that Th-POK expression in normal thymi was higher in the CD4+CD8 subset than in the CD4+CD8+ and CD4 CD8+ subsets. In thymomas, Th-POK expression in the CD4+CD8 subset was significantly lower than that in normal thymi, and was significantly correlated with the proportion of CD3+ cells in the CD4+CD8 subset. However, Th-POK expressions of the CD3+CD4+CD8+ and CD3+CD4+CD8 subsets were not impaired in thymomas compared to normal thymi. These results suggest that thymoma neoplastic epithelial cells can induce Th-POK expression similarly to the normal thymic epithelial cells. In addition, there was no significant difference in Runx3 expression between normal thymi and thymomas. Therefore, CD4/CD8 lineage commitment dependent on Th-POK and Runx3 system seems to be working even in the neoplastic environment formed by human thymomas.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

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