Identification of Antibody Epitopes within the CB-11 Peptide of Type II Collagen.: II. Computer Modelling Studies of Peptides and the Interpretation of Epitope Scanning Results

Abstract

Computer modelling techniques were used to investigate the structure of 8-mers from the CB-11 peptide of bovine type II collagen which were recognised by sera from rats which had previously been injected with bovine type II collagen. It was discovered that all the hydrophobic peptides recognised by the rat sera were predicted to have collagenous-like secondary structures. The primary structure of the 8-mers which were recognised was also compared against the sequences in the OWL protein sequence database. The combined results of the computer modelling and sequence analysis suggested that the sequence Gly-Pro-Gly-Phe-Pro is a minimal B cell epitope of the CB-11 fragment of bovine type II collagen.

Key Words:

• Type II collagen
• CB-11 peptide
• antibodies
• arthritis
• epitope scanning
• molecular modelling