A New Murine Model of Autoimmune Orchitis Induced by Immunization With Viable Syngeneic Testicular Germ Cells Alone.: II. Immunohistochemical Findings of Fully-Developed Inflammatory Lesion

Abstract

Previous studies demonstrated that experimental autoimmune orchitis (EAO) was produced in C3H/He mice with very high incidence by two or three subcutaneous injections of viable syngeneic testicular germ cells, without the use of any adjuvants or immunopotentiators and that the disease induced was characterized by a complete lack of epididymitis despite a definite orchitis with hypospermatogenesis. In this report, immuno-histochemical characterization of immune cells in the fully-developed orchitic lesion was carried out using monoclonal antibodies and immunoperoxidase staining. Thy-1.2⁺ cells, Mac-l⁺ cells, B220⁺ cells and cytoplasmic Ig-bearing cells in the lesion were estimated to be approximately 30, 15, 20 and 30% of all inflammatory cells, respectively. Major phenotype of T cells in the lesion was CD4⁺ (approximately 85%) with the remainder (approximately 15%) being CD8⁺. The percentages of cytoplasmic IgG-, IgA-and IgM-bearing cells were estimated as approximately 35, 60 and 5% of all cytoplasmic Ig-bearing cells, respectively. Deposits of immunoglobulins and third component of complement were identified on the basement membrane of the seminiferous tubules, interstitium between the tubules, vessel endothelium and degenerated germ cells in the lesion. Circulating antibodies directed against the acrosomal portion of germ cells were detected in IgG and IgM classes but not in IgA class. Inflammatory cells (including macrophages, B cells and, probably, activated T cells) in the lesion were Ia⁺, but Leydig cells, Sertoli cells and germ cells did not stain for la at all.

Key Words:

• Autoimmune orchitis
• immunohistochemistry
• testis