Naturally Occurring Anti-Idiotypic Antibodies to Anti-Phosphotyrosine in Systemic Lupus Erythematosus Interact with SRC-Homology 2 Domains

Maria Stefanescu Department of Immunology, Cantacuzino Institute, Bucharest, Romania

Adrian Onu Department of Immunology, Cantacuzino Institute, Bucharest, Romania

Cristiana Matache Department of Immunology, Cantacuzino Institute, Bucharest, Romania

Francisco Ramos-morales Institut Cochin de Génétique Moléculaire, U363 INSERM, Hopital Cochin, Paris, France

Siegmund Fischer Institut Cochin de Génétique Moléculaire, U363 INSERM, Hopital Cochin, Paris, France

Geza Szegli Department of Immunology, Cantacuzino Institute, Bucharest, Romania

Abstract

We have recently identified in SLE sera naturally occurring anti-idiotypic antibodies against antiphosphotyrosine antibodies. Analysis of immunochemical properties of these anti-idiotypic antibodies suggest that they are of beta/gamma type mimicking the antigen.

The interaction between these anti-idiotypes and SH2 domains of various fusion proteins was analysed by immunoprecipitation and immunoblotting.

Our data demonstrate that these anti-idiotypic antibodies specifically bind SH2 domains, with the highest affinity for SH2 domain of lck protein tyrosine kinase. The significance of this interaction is discussed.

Key Words:

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Naturally Occurring Anti-Idiotypic Antibodies to Anti-Phosphotyrosine in Systemic Lupus Erythematosus Interact with SRC-Homology 2 Domains

Information for

Open access

Opportunities

Help and information

Naturally Occurring Anti-Idiotypic Antibodies to Anti-Phosphotyrosine in Systemic Lupus Erythematosus Interact with SRC-Homology 2 Domains

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature