Peripheral Blood in Sjögren's Syndrome Does Not Contain Increased Levels of T lymphocytes Reactive with the Recombinant Ro/SS-A 52 kD and La/SS-B 48 kD Autoantigens

Abstract

Patients with Sjögren's syndrome (SS) frequently have anti-Ro/SS-A and anti-La/SS-B autoantibodies. The aim of this study was to investigate if these patients have peripheral blood lymphocytes (PBL) secreting IFN-γ after short-term cultivation in the presence of Ro/SS-A and La/SS-B antigens. The frequency of PBL secreting IFN-γ was examined in 12 SS patients and 11 healthy controls. The enzyme-linked immunospot (ELISPOT) assay was performed after 48 hours cultivation of PBL in the presence of recombinant Ro 52 kD protein or recombinant La 48 kD protein. The number of unstimulated IFN-γ secreting cells in the SS patient group was not significantly different from that of the control group. Moreover, no increase in the number of IFN-γ secreting cells after Ro/SS-A and La/SS-B stimulation was detected in the two groups. Thus, T cells reactive with the recombinant Ro 52 kD and La 48 kD proteins do not occur with any increased frequency in peripheral blood of SS patients.

Key Words:

• Sjögren's syndrome (SS)
• Ro/SS-A
• La/SS-B
• T lymphocytes