Different bacteria species lipopolysaccharide co-exposure with Pseudomonas exotoxin A on multiple organ injury induction

Abstract

The present study investigated the effect of different bacterial species lipopolysaccharide plus Pseudomonas exotoxin A (LPS/PEA) on the induction of multiple organ injury (MOI). Rats were injected with various LPS from Salmonella (SAE, SAT), E. coli (EB4, EB5), or P. aeruginosa (PAL) and PEA showed a greater mortality in the SAE/PEA and SAT/PEA groups. Histological alterations, serum enzymes, and cytokines changes were severer in the SAE/PEA group than the EB4/PEA or PAL/PEA group. EB4/PEA and PAL/PEA failed to induce MOI, even at the LPS doses increased up to 2–4- and 4–8-fold, respectively. Rats co-treated with Salmonella lipid A/PEA developed severer MOI than the E. coli lipid A/PEA. The results indicated the critical roles of MOI induction, which were related to LPS derived from appropriate bacterial species.

Keywords::

• Cytokines
• endotoxin unit
• lipopolysaccharide
• multiple organ injury
• pseudomonas exotoxin A
• TUNEL

Acknowledgments

The authors are grateful to Chieh-Yu Huang and Yen-Hsun Tseng for technical assistance. This study was supported in part by Grant NSC 93-2313-B-492-001 from the National Science Council, Taiwan.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.