Chemical characterization of various fractions of leaves of Cinnamomum tamala Linn toward their antioxidant, hypoglycemic, and anti-inflammatory property

Abstract

Cinnamomum tamala (CT) Linn. (Lauraceae) is a component of Indian spices and is also used as antidiabetic agent. Its antioxidant potential was reported by other authors and recently our group has reported its immune-suppressive property. Here, CT leaves were extracted with methanol (CTT) and also with various organic solvents of increasing polarity such as hexane (CTH), ethyl acetate (CTEA), and methanol (CTE) in successive manner by continuous Soxhlet extractor. The active compounds of CTT and CTH were analyzed by GC-MS and most abundant compound (eugenol) was further analyzed by HPLC. These extracts were tested for their free radical (FR) scavenging potential for different FR species, hypoglycemic potential on alloxan-induced hyperglycemia, and anti-inflammatory property in carrageenan-induced paw edema and lipopolysaccharide (LPS)-induced nitric oxide (NO) production in rat peritoneal macrophage culture. Result showed that CTH was the most active fraction toward these parameters, which was parallel to its eugenol and total phenolic content concentration.

Keywords::

• Cinnamomum tamala
• lauraceae
• free radicals
• anti-inflammatory
• eugenol

Acknowledgements

The authors are thankful to CSIR for financial assistance through its funded project. The GC-MS was carried out with courtesy of Dr Sheo Chand Chaurasia, Scientist H in CCCM Hyderabad.

Declaration of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.