Immunoresponsiveness in Chronic Hepatitis C Patients: Correlation Between Tissue and Serum Findings

Abstract

In the present study, intrahepatic CD8⁺ lymphocyte infiltrates as well as HLA class I and CD54 (ICAM-1) antigen expression at both tissue and serum levels were evaluated in 54 untreated patients with chronic hepatitis C stratified on the basis of histological diagnosis (Chronic Persistent Hepatitis/Chronic Lobular Hepatitis-CPH/CLH-and Chronic Active Hepatitis-CAH-: 22 and 32 subjects, respectively). The relationships between soluble HLA-I (sHLA-1) and ICAM-1 (sICAM-1) serum levels and their membrane-bound counterparts, CD8⁺ liver infiltration and serum alanine aminotransferase (ALT) were also studied.

A strong HLA-I and CD54 tissue expression, associated to the presence of CD8⁺ cell infiltrates in necro-inflammatory areas, and elevated sHLA-1 and sICAM-1 serum amounts were observed in all patients. At the same time, no difference was found at tissue level between the two groups of patients with respect to the mean scores of HLA-I and CD54 expression, while CAH subjects displayed a significantly higher CD8 periportal and lobular reactivity in comparison to the other subset. Serological assays outlined higher values of circulating HLA-I molecules in CPH/CLH patients and higher sICAM-1 levels in the CAH group. Finally, a negative correlation was found betweeen sHLA-1 and ALT in CAH subjects while, in all patients, sICAM-1 positively correlated with both CD8 tissue infiltration and ALT.

Our findings confirm the occurrence of an immune activation status during chronic hepatitis C and suggest that sHLA-1 molecules might play a down-modulating role on immunoresponsiveness of these patients.