An Assessment of Prolonged Reactivity of Seven Monoclonal Antibodies Against CX-1 Tumor Xenografts Using A Hand-Held Gamma-Detecting Probe

Abstract

The biodistribution and kinetics of 7 monoclonal antibodies (MAb) with known reactivity against CX-I tumor were examined over 21 days using a hand-held gamma-detecting probe (Neoprobe system). Twenty-eight irnmuno-deprived (athymic) nude mice implanted with human colon adenocarcinoma CX-1 xenografts were injected intraperitoneally with 50 µCi of¹²⁵I-labeled antibodies (4 mice/antibody). Of the 7 monoclonal antibodies, 4 were anti-CEA (MA, MB, MC, and MD), 2 were anti-TAG 72 (B72.3 NCI and B72.3 fermented) and one was anti-colorectal cancer (17-1A). Daily probe counts were recorded in duplicate over the tumor site and the contralateral nontumor site (background), and tumor-to-background (Tu/Bkg) ratios were calculated. Animals were sacrificed on day 21, and blood, heart, liver, spleen, lungs, kidneys, intestine, muscle, and the tumor were removed for gamma well counting. All antibodies identified the tumor as early as 24 h postinjection and speciJic tumor localization improved over time. Patterns of prolonged tumor binding varied considerably from one antibody to another, although all but one (MB) showed continuously increasing TulBkg ratios. These data indicate progressive clearance of the antibodies from the background tissue and a persistence of labeled MAb activity in tumor resulting in improved tumor localizution with increasing postinjection time.