Evaluation of Models, Methodologies, Devices, and New Surgical Concepts: Catheterization of the Subarachnoid Space in Rabbits Using a Vascular Access Port

Abstract

A surgical technique allowing chronic access to the subarachnoid space in unanesthetized rabbits was devised using the Vascular Access Port system. Instrumentation provides the investigator with a method of measuring intracranial pressure and sampling cerebrospinal fluid in awake rabbits. The catheter is introduced into the dorsal cervical subarachnoid space at the C4-C5 intervertebral space via dorsal laminectomy and passed cranially into the cisterna magna. The access port is subcutaneously implanted in the dorsal thoracic region between the scapulas. This system reduces the potential for infection, is not easily removed by the animal, and is relatively easy to access with minimal discomfort for the animal.

Key Words:

• subarachnoid space
• cerebrospinal fluid
• rabbit
• catheterization