Hemodynamic Changes in Blood Flow Through the Denervated Liver in Pigs

Abstract

We investigated the effects of liver denervation on hemodynamic circulation in seven anesthetized pigs. Simultaneous measurements of the hepatic artery and portal vein were performed with an ultrasound Doppler flow meter before and after liver denervation. Neither resting systemic nor hepatic hemodynamics changed following liver denervation. However, temporary occlusion of the portal vein resulted in a significant increase in hepatic artery flow in the innervated liver (from 123 ± 15 ml/min to 177 ± 17 mu/min, p <. 01), whereas, in the denervated liver, a significant decrease was observed (from 128 ± 11 ml/min to 106 ± 19 mu/min. p >. 05). Thus, the reciprocity between the hepatic artery and portal vein in the innervated liver disappeared in the denervated liver. The absence of an increase in the hepatic artery flow during portal vein occlusion might intensify symptoms of portal vein thrombosis in liver transplantation. In the denervated liver, a significant decrease also occurred in systolic blood pressure and central venous pressure from 1 to 3 min after portal vein occlusion. Since the liver plays a crucial role in the maintenance of cardiovascular homeostasis during blood loss, it is likely that denervation at the porta hepatis induced a lack of vasoconstriction in the portal territory. Liver denervation might further exacerbate this response to hypotension. The current study confirms that the hepatic nerves play an important role in hepatic arterial and portal venous interactions aimed at maintaining a constant blood flow through the liver. We also suggest that the hepatic nerves are important for cardiovascular homeostasis.

Key Words:

• liver blood flow
• denervation
• reciprocity
• liver transplantation