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Inhalation Toxicology
International Forum for Respiratory Research
Volume 24, 2012 - Issue 8
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Research Article

Exposure to diesel exhaust upregulates COX-2 expression in ApoE knockout mice

, , , , &
Pages 518-527 | Received 06 Mar 2012, Accepted 19 May 2012, Published online: 02 Jul 2012
 

Abstract

Introduction: We have shown that diesel exhaust (DE) inhalation caused progression of atherosclerosis; however, the mechanisms are not fully understood. We hypothesize that exposure to DE upregulates cyclooxygenase (COX) expression and activity, which could play a role in DE-induced atherosclerosis.

Methods: ApoE knockout mice (30-week old) fed with regular chow were exposed to DE (at 200 µg/m3 of particulate matter) or filtered air (control) for 7 weeks (6 h/day, 5 days/week). The protein and mRNA expression of COX-1 and COX-2 were evaluated by immunohistochemistry analysis and quantitative real-time PCR, respectively. To examine COX activity, thoracic aortae were mounted in a wire myograph, and phenylephrine (PE)-stimulated vasoconstriction was measured with and without the presence of COX antagonists (indomethacin). COX-2 activity was further assessed by urine 2,3-dinor-6-keto PGF level, a major metabolite of prostacyclin I2 (PGI2).

Results: Immunohistochemistry analysis demonstrates that DE exposure enhanced COX-2 expression in both thoracic aorta (p < 0.01) and aortic root (p < 0.03), with no modification of COX-1 expression. The increased COX-2 expression was positively correlated with smooth muscle cell content in aortic lesions (R2 = 0.4081, p < 0.008). The fractional changes of maximal vasoconstriction in the presence of indomethacin was attenuated by 3-fold after DE exposure (p < 0.02). Urine 2,3-dinor-6-keto PGF level was 15-fold higher in DE group than the control (p < 0.007). The mRNA expression of COX-2 (p < 0.006) and PGI synthase (p < 0.02), but not COX-1, was significantly augmented after DE exposure.

Conclusion: We show that DE inhalation enhanced COX-2 expression, which is also associated with phenotypic changes of aortic lesion.

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