Secretion pathway of liver IGF-1 via JAK2/STAT3 in chick embryo under the monochromatic light

Abstract

This study reveals mechanism of monochromatic light on the IGF-1 secretion of chick embryo liver. The chick embryos were incubated and exposed to continuous red, green, blue light or a dark environment. Compared to other light-treated groups, green light increased IGF-1 and melatonin concentrations both in plasma and liver, and Mel1a, Mel1b and Mel1c receptors expressions in liver but decreased p-JAK2, p-STAT3 and ROS in liver. IGF-1 had a positive correlation with melatonin, but a negative relevance with p-JAK2 and p-STAT3. In vitro, the IGF-1 level in the hepatocyte supernatant was enhanced by melatonin with lower p-JAK2/p-STAT3 and ROS levels, which was suppressed by Mel1c antagonist but not Mel1a/Mel1b or Mel1b antagonists. AG490 (JAK/STAT inhibitor) promoted role of melatonin-Mel1c modulated IGF-1 secretion. These results suggest the antioxidant effect of melatonin mediated the green light-enhanced IGF-1 secretion of chick embryo liver through Mel1c receptor to inhibit the JAK2/STAT3 pathway.

• Monochromatic light
• IGF-1
• JAK2/STAT3
• chick embryo
• liver

Declaration of interest

The authors of the manuscript have no conflicts of interest to declare.

This work was supported by the National High-tech Research and Development Projects (863) (No. 2013AA102306), the Chinese National Natural Science Foundation (31172277, 31272516 and 31472157), and the Chinese Specialized Research Fund for the Doctoral Program of Higher Education (20100008110022).