Expansion of Hemopoietic Activity in Long-Term Culture of Human Bone Marrow by c-kit Ligand (Stem Cell Factor)

Abstract

The response of hemopoietic activity in long term culture of human bone marrow (LTMC) to exogenous c-kit ligand has been investigated. Addition of c-kit ligand as recombinant Stem Cell Factor after the adherent stromal cell layer had become confluent failed to increase the content of hemopoietic cells in the culture system. Production of hemopoietic progenitors and differentiated progeny was on the other hand amplified by addition of c-kit ligand at regular intervals from the initiation of the culture, and resulted in net formation of these cells in the culture system. The characteristics of the response were consistent with consequences of increased survival of hemopoietic progenitors during a vulnerable initial phase in which the availability of endogenous c-kit ligand was limited by the initially low number of stromal cells. The increase in differentiated progeny was essentially due to increased formation of neutrophil series, and the lack of increased basophil production under these circumstances presumably reflected the nature of the contributory effects of endogenously generated growth regulatory molecules which acted in a synergistic manner with added c-kit ligand.

Key Words:

• c-kit ligand
• long-term marrow culture
• hemopoiesis
• stem cellsw