Gentamicin Encapsulation in Liposomes: Factors Affecting the Efficiency

Abstract

Gentamicin was encapsulated in small unilamellar vesicles (SUV), multilamellar vesicles (ML V), and dehydration-rehydration vesicles (DRV). The encapsulation efficiency of the three liposomal preparations was compared. The values corresponding to DRV are ten to fifteen times greater than those for MLV or SUV. For dehydration-rehydration vesicles, the influence of lipid constituents, mixtures of PC, PS, SA, Chol, in encapsulation efficiency was also determined. The stability of the DRV preparations yielding the maximum encapsulation value, as a function of osmotic gradients and incubated in the presence of blood, was determined.