T_reg Lymphocytes in Autoimmune Uveitis

Abstract

Purpose: To evaluate circulating CD4⁺CD25⁺ regulatory T-cell populations in patients with autoimmune uveitis and to assess whether T-regulatory cell populations correlate with clinical features.

Methods: Sixty-four patients with noninfectious uveitis were enrolled. Following isolation and purification of peripheral blood mononuclear cells, Tregs were analyzed by assessing FOXP3 expression on T cells with an APC anti-human FOXP3 staining kit. Samples were simultaneously stained with anti-CD25-PeCy7, anti-CD3-FITC, anti-CD4-PercP, and anti-CD152 (CTLA-4)-PE monoclonal antibodies.

Results: A decrease in CD4⁺CD25⁺FOXP3⁺ T cells was detected in patients with active uveitis compared with healthy controls (p < .05). In patients achieving clinical remission, the percentage of CD4⁺CD25⁺FOXP3⁺ T cells increased up to values comparable to those of healthy controls, in step with an increased expression of CTLA4.

Conclusion: Therapy of autoimmune uveitis results in the increase of T-regulatory lymphocyte population and restoration of their functional state. These changes are likely to contribute to the patients' clinical improvement or disease regression.

KEYWORDS::

• Cytotoxic T lymphocyte-associated antigen (CTLA)-4
• immunomodulatory therapy
• interleukin-2 receptor α chain (CD25)
• noninfectious uveitis
• standardization of uveitis nomenclature
• T regulatory cells

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.