Classic PCOS phenotype is not associated with deficiency of endogenous vitamin D and VDR gene polymorphisms rs731236 (TaqI), rs7975232 (ApaI), rs1544410 (BsmI), rs10735810 (FokI): a case–control study of lower Silesian women

Abstract

Context: The role of endogenous vitamin D and vitamin D receptor (VDR) gene polymorphism in polycystic ovary syndrome (PCOS) is still controversial.

Objective: The objective of this study was to investigate for the first time in women with “classic” PCOS phenotype and healthy controls the role of the serum endogenous vitamin D level and VDR gene polymorphisms in PCOS etiology.

Design: Ninety-two women with “classic” PCOS phenotype and 85 controls from lower Silesia with comparable body mass index (BMI) were studied. In all women the waist circumference, android/gynoid fat deposit, parameters of lipid and glucose metabolism, testosterone, free androgen index, sex hormone binding globulin (SHBG) and vitamin D were evaluated. Also, VDR gene polymorphisms rs731236, rs7975232, rs1544410 and rs10735810 were assessed.

Results: Serum vitamin D levels in both groups were comparable. Also high, comparable frequencies of hypovitaminosis and vitamin D deficiency in both groups were observed. Women with “classic” PCOS phenotype had statistically significantly higher values of all measured parameters, except serum SHBG and high-density lipoprotein (HDL)-cholesterol, which were lower. The frequency of VDR genotype polymorphism was also comparable in both groups.

Conclusions: For the first time, we show that endogenous vitamin D deficiency and VDR polymorphisms are not associated with homogeneous “classic” PCOS phenotype.

Chinese abstract

背景：内源性维生素D和维生素D受体基因多态性在PCOS中的作用扔有争议。

目的：本研究的目的是首次调查典型PCOS表型的女性患者和健康对照组内源性维生素D和维生素D受体基因多态性的血清水平在PCOS病因中的作用。

设计：92名典型PCOS患者和85名体重指数相匹配的西里西亚女性作为对照组进行研究。测量了所有女性的腰围,，臀部脂肪沉积，脂肪和糖代谢，睾酮，游离雄激素指数，性激素结合球蛋白和维生素D。此外还评估了维生素D受体基因多态性rs731236，rs7975232，rs1544410和rs10735810

结果：两组患者的血清维生素D水平进行了比较。发现两组患者维生素缺乏症和维生素D缺乏的发生率是高度匹配的。典型PCOS患者的所有测量参数除了血清性激素结合球蛋白和高密度脂蛋白-胆固醇较低之外，其他均显著升高，具有统计学意义。两组患者维生素D受体基因多态性的发生率也进行了比较。

结论：我们首次表明内源性维生素D缺乏和维生素D受体基因多态性与典型PCOS表型并不相关。

Keywords:

• PCOS
• VDR
• VDR polymorphism
• vitamin D

Declaration of interest

This research did not receive any specific grant from any funding agency in the public, commercial or not-for-profit sector. The authors declare that they have no conflict of interest.

Ethical approval

The procedures used were in accordance with the guidelines of the Helsinki Declaration on human experimentation, and Local University Ethical Committee permission was obtained.

All study participants gave written informed consent for participation in the project.