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Papers Presented at the 2nd Workshop on Radiation and Multidrug Resistance Mediated via the Tumour-Microenvironment

Co-localisation of hypoxia and perfusion markers with parameters of glucose metabolism in human squamous cell carcinoma (hSCC) xenografts

, , , , , , & show all
Pages 972-980 | Received 31 Mar 2009, Accepted 26 Jul 2009, Published online: 06 Nov 2009
 

Abstract

Purpose: To examine relationships between tumour hypoxia, perfusion and metabolic microenvironment at the microregional level in three different human squamous cell carcinomas (hSCC).

Materials and methods: Nude mice bearing FaDu, UT-SCC-15, and UT-SCC-5 hSCC were injected with pimonidazole hypoxia and Hoechst perfusion markers. Bioluminescence imaging was used to determine spatial distribution of glucose and lactate content in serial tumour sections. Metabolite levels were grouped in 10 concentration ranges. Images were co-registered and at each concentration range the proportion of area stained for pimonidazole and Hoechst was determined in 11–13 tumours per tumour line.

Results: The spatial distribution of metabolites in pimonidazole hypoxic and Hoechst perfused areas is characterised by pronounced heterogeneity. In all three tumour lines glucose concentration decreased with increasing pimonidazole hypoxic fraction and increased with increasing perfused area at the microregional level. A weak albeit significant positive correlation between lactate concentration and pimonidazole hypoxic fraction was found only in UT-SCC-5. Lactate concentration consistently decreased with increasing perfused area in all three tumour lines.

Conclusions: Both glucose consumption and supply may contribute to the microregional glucose levels. Microregional lactate accumulation in tumours may be governed by clearance potential. The extent of microregional hypoxia cannot be predicted from the lactate concentration indicating that both parameters need to be measured independently.

Acknowledgments

This investigation was supported by the Deutsche Forschungsgemeinschaft (Ba 1433/4-1, Ba1433/5-1 [to MB and DZ] and Mu 576/14-1, -2, -3, Mu 576/15-1 [to WMK]). The authors thank Mrs E. Wenzel, Mrs S. Balschukat, Mrs D. Pfitzmann, Mrs K. Schumann, and Mrs M. Oelsner for excellent technical assistance.

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

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