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Isolation of β-mannanase from Cocos nucifera Linn haustorium and its application in the depolymerization of β-(1,4)-linked d-mannans

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Pages 272-281 | Received 06 Aug 2009, Accepted 01 Oct 2009, Published online: 20 Apr 2010
 

Abstract

β-Mannanase was extracted from coconut (Cocos nucifera Linn) haustorium and purified through ammonium sulfate precipitation and sepharose 6B–lectin affinity chromatography. Coconut β-mannanase is an acidic protein with a pI of 3.75. The molecular mass of coconut β-mannanase (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) was found to be 44 kDa and was confirmed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The optimum temperature and pH for enzyme activity was 70°C and 5.2. The enzyme was used for the preparation of neutraceutical dietary supplement from galactomannans of guar gum and tender coconut kernel having a β-(1,4)-linked d-mannose backbone. Depolymerized guar gum has 92% of oligosaccharides with a degree of polymerization of 3 and 7. Tender coconut kernel has a degree of polymerization of 9–39 oligosaccharides along with disaccharides and trisaccharides. Hence this mannanase will be useful to depolymerize β-(1,4)-linked d-mannose polysaccharides from most plant sources to produce prebiotics in a cost-effective technique.

Acknowledgements

The authors are grateful to the Director, National Institute for Interdisciplinary Science and Technology (NIIST, CSIR), Trivandrum, India for providing the necessary facilities for the work.

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