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Original Article

Mushroom tyrosinase inhibitors from mung bean (Vigna radiatae L.) extracts

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Pages 358-361 | Published online: 01 Nov 2011
 

Abstract

A seventy percent ethanol from mung bean (Vigna radiatae L.) was extracted further with CH2Cl2, EtOAc and n-BuOH to afford four fractions: CH2Cl2-soluble, EtOAc-soluble, n-BuOH-soluble and residual extract fractions. When using l-3,4-dihydroxyphenylalanine as the substrate for mushroom tyrosinase, the EtOAc-soluble fractions showed the highest inhibitory activity. Two pure flavonoid compounds, vitexin and isovitexin, were isolated (using the enzyme assay-guided fractionation method) from the EtOAc-soluble fractions. Vitexin and isovitexin showed high inhibitory activities, with IC50 values of 6.3 and 5.6 mg/ml, respectively. This is the first study on the active compositions of azuki beans against mushroom tyrosinase.

Acknowledgement

This study was supported by the earmarked fund for Modern Agro-industry Technology Research System nycytx-018 (to Guixing Ren).

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

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