Organic and Inorganic Solute Transport in Renal and Intestinal Membrane Vesicles Preserved in Liquid Nitrogen

B. R. Stevens Department of Physiology, School of Medicine, University of California and Cedars-Sinai Medical Center, Los Angeles, CA

S. H. Wright Department of Physiology, School of Medicine, University of California and Cedars-Sinai Medical Center, Los Angeles, CA

B. S. Hirayama Department of Physiology, School of Medicine, University of California and Cedars-Sinai Medical Center, Los Angeles, CA

R. D. Gunther Department of Physiology, School of Medicine, University of California and Cedars-Sinai Medical Center, Los Angeles, CA

H. J. Ross Department of Physiology, School of Medicine, University of California and Cedars-Sinai Medical Center, Los Angeles, CA

V. Harms Department of Physiology, School of Medicine, University of California and Cedars-Sinai Medical Center, Los Angeles, CA

E. Nord Department of Physiology, School of Medicine, University of California and Cedars-Sinai Medical Center, Los Angeles, CA

I. Kippen Department of Physiology, School of Medicine, University of California and Cedars-Sinai Medical Center, Los Angeles, CA

E. M. Wright Department of Physiology, School of Medicine, University of California and Cedars-Sinai Medical Center, Los Angeles, CA

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Abstract

The transport of organic solutes (sugars, amino acids, and metabolic intermediates) and inorganic solutes (Na⁺/H⁺ exchange and Na⁺/SO = ₄ cotransport) in renal brush border and in intestinal brush border and basal lateral membrane vesicles is preserved when the vesicles are stored in liquid nitrogen. The preservation allows comparisons among transport systems of renal and intestinal cells obtained from the same animal.

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Organic and Inorganic Solute Transport in Renal and Intestinal Membrane Vesicles Preserved in Liquid Nitrogen

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Organic and Inorganic Solute Transport in Renal and Intestinal Membrane Vesicles Preserved in Liquid Nitrogen

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