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Abstract
Beef heart mitochondrial H+-ATPase (F1-F0) vesicles were prepared by lysolecithin extraction of ETPH. ATP-driven membrane potential was monitored indirectly by following absorbance changes of the potential-sensitive dye oxonol VI. The steady-state potential was discharged by oligomycin and/or Cd2+ (a dithiol reagent). At 13°C, the agents appeared to act synergistically; at 24°C the data were equivocal. When Cd2+ was added before energization, the membrane potential was markedly attenuated. Both effects of Cd2+ were inhibited by dithiothreitol. The activation energy for oligomycin-sensitive ATPase exhibited a discontinuity at 16°C. However, the temperature dependence of the rate of potential discharge by oligomycin showed no such discontinuity. The results are discussed in terms of the involvement of thiol groups in proton translocation and the thermotropic behavior of the membrane vesicles.