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Original Article

Active Uptake of Glutamate in Vesicles of Halobacterium salinarium

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Pages 1-17 | Published online: 09 Jul 2009
 

Abstract

Uptake of glutamate into vesicles of Halobacterium salinarium has been studied during respiration and in the nonrespiring state. Uptake requires respiration or a minimum gradient in NaCl, which is consistent with an Na+ symport mechanism for uptake, as proposed for H. halobium. By replacing KCl or NaCl by choline chloride, it has been possible to distinguish between the effects of gradients and/or absolute concentration effects of NaCl and KCl. Uptake depends on the concentration of KCl on the inside, but not on a gradient in KCl. This points to a role for K+ as a regulator of uptake rate, but not of total uptake.

The uptake of glutamate is not inhibited by a number of acids with similar chemical groups. Inhibition is, however, caused by D-glutamate. This indicates a specific transport site for glutamate. Parallel results are obtained for binding of glutamate to a Triton extract of the vesicle membrane. The variation in binding and uptake properties with the salt concentration is discussed with reference to transport kinetics.

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