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Original Article

Solubilization and Reconstitution of the Calcium Release Channel of Skeletal Sarcoplasmic Reticulum

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Pages 327-333 | Published online: 09 Jul 2009
 

Abstract

Fragmented heavy sarcoplasmic reticulum was solubilized by cholate, and proteins were subsequently fractionated by using diethylaminoethyl-cellulose (DEAE-cellu-lose) column chromatography. A fraction was collected in which proteins with molecular weights between 31,000 and 45,000 u were major components. This fraction, when incorporated into Ca2+-loaded liposomes, facilitated the Ca2+ efflux. The rate of efflux was regulated by the external Ca2+ concentration, reaching a maximum at 3 μ-M Ca2+. The Ca2+ efflux was suppressed by Mg2+.

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