A trans-dominant mutation in human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein gp41 inhibits membrane fusion when expressed in target cells

Summary

A recombinant vaccinia virus was used to express a mutation in the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein gp120-gp41. In this mutant protein, the second amino acid in the N-terminal region of gp41 has been converted from a hydrophobic valine residue to the polar glutamate. When recombinant vaccinia viruses encoding wild-type HIV-1 envelope glycoprotein Infect a lymphocyte cell line lacking CD4, the cells express the HIV-1 envelope glycoprotein gp120-gp41 and are able to fuse with a CD4⁴ T lymphocyte cell line. Cells expressing the mutant envelope glycoprotein are unable to fuse with CD4⁴ T lymphocytes. When both viruses infect CD4⁻ cells simultaneously, there is an inhibition of fusion to CD4⁺ cells with an increasing fraction of the virus encoding the mutated envelope glycoprotein. Interestingly, when the opposing, or CD4⁺ target cells are infected with the mutation-expressing virus, while CD4⁻ cells are infected with wild-type envelope-expressing virus, a similar inhibition of fusion is observed. This suggests that the mutated envelope glycoprotein does not need to reside in the same membrane as the wild-type protein it inhibits.

Key Words:

• Fusion
• HIV-1
• envelope glycoprotein
• CD4
• vaccinia virus