Assignment of the human serotonin 1F receptor gene (HTR1F) to the short arm of chromosome 3 (3p13-p14.1)

Jeanette Erdmann Institute of Human Genetics, University of Bonn, Wilhelmstr, 31, 53111, Bonn, Germany

Daphne Shimron-Abarbanell Institute of Human Genetics, University of Bonn, Wilhelmstr, 31, 53111, Bonn, Germany

Viji Shridhar Division of Hematology/Oncology, Department of Internal Medicine, Wayne State University School of Medicine, Detroit, MI, 48201, USA

David I. Smith Division of Experimental Pathology, Department of Laboratory Medicine and Pathology, Mayo Foundation, Rochester, MN, 55905, USA

Peter Propping Institute of Human Genetics, University of Bonn, Wilhelmstr, 31, 53111, Bonn, Germany

Markus M. Nöthen Institute of Human Genetics, University of Bonn, Wilhelmstr, 31, 53111, Bonn, Germany

Abstract

In the present study, we report the chromosomal localization of the human 5-HT_1F receptor gene (HTR1F) by the analysis of somatic cell hybrids. Based upon the HTR1F cDNA sequence, a primer set that reacted with human genomic DNA but not mouse or hamster genomic DNA was derived from the relatively non-conserved 5′-untranslated and coding region. Using monochromosomal hybrid cell lines of the NIGMS Mapping Panel 2 we localized the HTR1F to human chromosome 3. To confirm the localization on chromosome 3 and to further sublocalize the HTR1F gene, a set of human cell hybrids regionally separating chromosome 3 into 7 regions was similarly analysed. Analysis of this regional panel showed that the HTR1F gene was located proximal to the 3p14.1 breakpoint in hybrid APH14 and distal to the breakpoint in 3p13 in hybrid APH13. This localizes the HTR1F gene to human chromosome 3p13-p14.1.

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Assignment of the human serotonin 1F receptor gene (HTR1F) to the short arm of chromosome 3 (3p13-p14.1)

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Assignment of the human serotonin 1F receptor gene (HTR1F) to the short arm of chromosome 3 (3p13-p14.1)

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