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Abstract
The influence of various reaction parameters, such as alcohol-to-substrate ratio, enzyme-to-substrate ratio, solvent and temperature, on the enzymatic preparation of a series of novel medium- and long-chain esters of 2-oxoglutaric acid has been evaluated. Among the tested lipases, those from Candida antarctica and Carica papaya appeared to be the best catalysts. Mild reaction conditions and low environmental impact make the biocatalytic procedure a convenient way to prepare the reported products, which are potential fat substitutes in the food industry.
Acknowledgements
The authors gratefully acknowledge M. M. Rivero for his assistance with GC analysis.
Declaration of interest: The authors thank UBA X010, CONICET PIP 112-200801-00801/09 and ANPCyT PICT 2005-32735 for partial financial support. A.B. is a Research Member of CONICET. The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.