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Abstract
Ultrasound has been successfully used to improve the performance of Mucor miehei lipase (MML) in enantioselective transesterification of N-hydroxymethyl vince lactam with vinyl valerate as acyl donor in methyl tert-butyl ether. The effects of reaction conditions have been investigated. Under the optimum conditions (ultrasound power, 175 W; temperature, 30°C; water activity, 0.22; pH, 7.0), the enzyme activity of MML was 93.7 μmol/g/min and the enantioselectivity (E value) was 68.2. The results also indicated that the free enzyme was stable under low power ultrasound conditions.
Acknowledgements
The authors thank Dr. Zhang Chuang (College of Chemistry, Jilin University) for providing the racemic N-hydroxymethyl vince lactam. We also thank Dr. Zhang Dali for providing the help in ultrasound irradiation experiments.
Declaration of interest: The authors report no declarations of interest. The authors alone are responsible for the content and writing of the paper.
The authors are grateful for the financial support from National Natural Science Foundation of China (No. 21172093, 31070708 and 30870539) and Scientific Research Project of Liaoning Educational Committee (general project No. L2012431).