Kinetic Equations for L-Phenylalanine Production by a Hyperproducing Mutant of Escherichia Coli

Abstract

Using a set of kinetic equations, which take account of the effect of both intra-and extracellular phosphate concentrations, the batch culture production of L-phenylalanine by a hyperproducing mutant of E. coli has been described. Good agreement has been found between the predicted results and experimental data for cell growth, glucose uptake, phosphate uptake and L-phenylalanine production for initial glucose concentrations of 16 and 26 g. L^-1. The kinetic model is applicable to conditions for which no substrate inhibition or product inhibition are likely to occur.

Key Words:

• L-phenylalanine production
• E. coli
• hyperproducing mutants
• kinetic model
• phosphate concentration