Kinetic Analysis and Simulation Studies for Lipase-Catalysed Resolution of Racemic 2-Methyl-1-Pentanol

Abstract

The lipase-catalysed optical resolution of a racemic mixture of 2-methyl-l-pentanol by transesterifica-tion using vinyl acetate as acyl donor has been studied experimentally. A mechanistic model has been developed for the double-substrate reaction sequence treating both enantiomers as competing substrates. The model is based upon a ping-pong mechanism with alternative substrates involving an acyl-enzyme intermediate. The kinetic constants of the model have been evaluated using initial rate experiments and nonlinear regression analysis. The model successfully predicts the evolution of the enantiomeric excess of substrate (ee_R) and the degree of conversion with time for batch experiments with various initial concentrations of vinyl acetate and (R,S)-2-methyl-1-pentanol. Furthermore, the rate equations have been used to theoretically study the dynamic progression of a continuous enzyme-catalysed resolution process. The enantiomeric excess as a function of conversion for different process configurations is discussed. It is found, that the maximum attainable ee_R is strongly dependent on the residence time distribution of the continuous reactor and is rather low for a continuous stirred tank reactor (CSTR) due to competitive inhibition effects.

Key Words:

• Enzyme kinetics
• mathematical modelling
• optical resolution
• lipase
• 2-methyl-1-pentanol