Sage Transcript Profiles in Cultured Human Fetal Fibroblasts, WI-38

Stanislav Shklyaev Department of Nature Medicine, Atomic Bomb Disease Institute, Nagasaki University School of Medicine, Nagasaki, 852-8523, Japan, (In final form June 22, 1999)

Hiroyuki Namba Department of Nature Medicine, Atomic Bomb Disease Institute, Nagasaki University School of Medicine, Nagasaki, 852-8523, Japan, (In final form June 22, 1999)Correspondence[email protected]

Takeshi Hara Department of Nature Medicine, Atomic Bomb Disease Institute, Nagasaki University School of Medicine, Nagasaki, 852-8523, Japan, (In final form June 22, 1999)

Akira Ohtsuru Department of Nature Medicine, Atomic Bomb Disease Institute, Nagasaki University School of Medicine, Nagasaki, 852-8523, Japan, (In final form June 22, 1999)

Shunichi Yamashita Department of Nature Medicine, Atomic Bomb Disease Institute, Nagasaki University School of Medicine, Nagasaki, 852-8523, Japan, (In final form June 22, 1999)

Abstract

Serial analysis of gene expression (SAGE) allows us to analyze the profile of genes expressed in human lung fibroblast cell line WI-38. Manual sequencing of approximately 10,000 transcripts derived from WI-38 cells revealed 1025 genes expression among of which 431 tags matched GenBank entries, whereas 594 corresponded to novel previously undescribed transcripts. 7SL RNA (7L7) 5′-truncated pseudogene and the gene with no match (tag: TCCCTAGCT) were found the most abundant among all the analyzed genes (10.2 and 4.6%) respectively). The expression pattern of the genes coding for cytoskeletal proteins, extracellular matrix components (ECM) and cytokines has been obtained. The results here demonstrate the unique gene expression pattern in human WI-38 fibroblast cell line and support a possibility of useful application of SAGE method for differential expression analysis.

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