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Abstract
The β-actin genes of two species of southern top mouth minnow and the common fat minnow from the family Cyprinidae were amplified and characterized. The exon and intron boundaries of the β-actin gene matched the consensus acceptor and donor sites according to the GT/AG rule. The amino acid sequence identity between the two species is 100.09, while there is a lower degree of homology with other species in the range of 98.7% to 100.0%. The expression of the β-actin gene was confirmed in liver.