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Abstract
Acidic ribosomal phosphoprotein PO has been widely used as an estradiol-independent housekeeping gene. Expression of acidic ribosomal phosphoprotein PO in canine prostate was detected and canine specific cDNA was cloned. Total cellular RNA was isolated from normal young (5 month-old) canine prostate tissue. Total cDNAs were synthesized from total cellular RNAs by reverse transcription assay. Primers used in polymerase chain reaction were designed from published human ARPO cDNA sequences and utilized to amplify 562 base-pair (bp) canine acidic ribosomal phosphoprotein PO cDNA. Nucleotide sequences of canine ARPO cDNA clones were determined on both strands. Canine ARPO cDNA and their deduced amino acid sequences share very high homology to ARPO orthologs from other vertebrate species including human, mouse, rat, chicken, and bovine.