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DNA Sequence Volume 11, 2000 - Issue 1-2
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Original Article

Isolation of Rat Mitochondrial Transcription Factor A (r-Tfam) cDNA

Hidetoshi Inagaki Department of Chemistry, National lndustrial Research Institute of Nagoya, 1 Hirate-Cho, Kita-ku, Nagoya, 462–8510, JapanCorrespondence[email protected]
,
Toshihiko Hayashi Faculty of Agriculture, Meijyo University, 1–501 Shiogamaguchi, Tenpaku-ku, Nagoya, 468–8502, Japan
,
Yuichi Matsushima Graduate Program for Regulation of Biological Signals, Graduate School of Bioagricultural Science, Nagoya University, Chikusa, Nagoya, 464–8601, Japan
,
Kong Hua Lin Department of Biology, University of Victoria, Victoria, BC, Canada
,
Sumio Maeda Department of Chemistry, National lndustrial Research Institute of Nagoya, 1 Hirate-Cho, Kita-ku, Nagoya, 462–8510, Japan
,
Shigeyuki Ichihara Faculty of Agriculture, Meijyo University, 1–501 Shiogamaguchi, Tenpaku-ku, Nagoya, 468–8502, Japan
,
Yasuo Kitagawa Graduate Program for Regulation of Biological Signals, Graduate School of Bioagricultural Science, Nagoya University, Chikusa, Nagoya, 464–8601, Japan
&
Takao Saito Department of Chemistry, National lndustrial Research Institute of Nagoya, 1 Hirate-Cho, Kita-ku, Nagoya, 462–8510, Japan
 show all
Pages 131-135 | Received 26 Aug 1999, Published online: 11 Jul 2009
 
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Abstract

We have isolated rat mitochondrial transcription factor A (Tfam; formerly known as mtTFA) cDNA clones from a rat cerebellum cDNA library using human Tfam cDNA as a probe. The deduced amino acid sequence of r-Tfam shows 62% and 89% overall identity to human and mouse Tfam, respectively. We also show the presence of two r-Tfam isoforms in testis as for mouse. Our findings suggest that the mechanisms underlying transcription of mitochondrial genes are conserved among rat, mouse, and human.

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