Molecular Cloning and Sequence Analysis of the Manganese Catalase Gene from Thermoleophilum album NM

Abstract

The manganese catalase gene (mnct) from Thermoleophilum album NM, a thermophilic bacterium, was cloned and its nucleotide sequence was analyzed. The gene consists of 885 bp (65.4% GC content) encoding 294 amino acids with a molecular mass of 32,500 Da. The deduced amino acid sequence shows similarities to those of Thermus species strain YS 8-13 (a thermophilic bacterium) and Bacillus halodurans (an alkaliphilic bacterium) with 61 and 54% identities, respectively.

Key Words:

• Thermoleophilum album NM
• Manganese catalase
• Thermostable enzyme
• Nucleotide sequence