Molecular cloning and sequencing of Australian black bream Acanthopagrus butcheri and barramundi Lates calcarifer fish growth hormone cDNA using polymerase chain reaction

Wayne Knibb Department of Biochemistry, University of Adelaide, Box 498, G.P.O, Adelaide, 5001, Australia

Allan Robins Department of Biochemistry, University of Adelaide, Box 498, G.P.O, Adelaide, 5001, Australia

Lesley Crocker Department of Biochemistry, University of Adelaide, Box 498, G.P.O, Adelaide, 5001, Australia

Janelle Rizzon Department of Biochemistry, University of Adelaide, Box 498, G.P.O, Adelaide, 5001, Australia

Andrew Heyward Department of Obstetrics and Gynaecology, University of Adelaide, Box 498, G.P.O, Adelaide, 5001, Australia

Julian Wells Department of Biochemistry, University of Adelaide, Box 498, G.P.O, Adelaide, 5001, Australia

Abstract

The complementary DNA (cDNA) encoding the preprotein growth hormone (pre-GH) from two Australian marine fish species, namely Acanthopagrus butcheri and Lates calcarifer, have been isolated, cloned and sequenced. The sequences were amplified from reverse transcribed total RNA of whole brains using Polymerase Chain Reaction (PCR) and oligonucleotide primers corresponding to the 5′ and 3′ regions of Pagrus major. Use of PCR offers a rapid method of isolating fish GH cDNA sequences for commercial and taxonomic applications. Sequence comparison indicates a high degree of conservation for GH cDNAs within the family Sparidae.

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Molecular cloning and sequencing of Australian black bream Acanthopagrus butcheri and barramundi Lates calcarifer fish growth hormone cDNA using polymerase chain reaction

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Molecular cloning and sequencing of Australian black bream Acanthopagrus butcheri and barramundi Lates calcarifer fish growth hormone cDNA using polymerase chain reaction

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