Sequence of a HeLa cDNA provides the DNA binding domain and carboxy terminus of HE47: a human Helix—Loop—Helix protein related to the enhancer binding factor E47

Abstract

In order to identify cDNA encoding regulators of gene expression, a HeLa λgt11 expression library was screened with a DNA segment containing multiple copies of a sequence spanning the AP4 site in the simian virus 40 control region. We identified a partial cDNA encoding HE47, a sequence-specific DNA binding protein. The HeLa clone overlaps with a previously reported human B-cell partial cDNA encoding E47. The HeLa cDNA includes the HE47 DNA binding domain, its carboxy terminus, and the 3′ untranslated region of its mRNA.

Key Words:

• eukaryotic DNA binding proteins
• human regulatory proteins
• transcription factors
• upstream pseudo-gene sequence
• repetitive DNA