The nucleotide sequence between genes 31 and 30 of bacteriophage T4

Abstract

The nucleotide sequence of the 2994 bp T4 phage DNA fragment between genes 31 and 30 is presented. The fragment contains 7 complete open reading frames in the direction of early transcription and two early promoters, P_E128.6 and P_E128.2, which we show to cause difficulties in cloning DNA from this genomic region. Our data complete the nucleotide sequence and the organization of genes in the genomic region between T4 genes 31 and 30.

Key Words:

• ORFs
• PCR
• promoters
• terminators