Nucleotide sequence of the internal transcribed spacers and 5.8s region of ribosomal DNA in Phus pinea L

Abstract

The nucleotide sequence of the first internal transcribed spacer (ITSI) belonging to different ribosomal RNA genes from Pinus pinea are reported. The analyzed lTSl can be distinguished on the basis of their length, being one 2631 bp and the other 271 bp long. Nucleotide comparison of these regions did not show appreciable sequence homology. The larger ITS1 contains five tandem arranged subrepeats with size ranging between 219 bp and 237 bp. The nucleotide sequence of the 5.8s and the ITS2 regions belonging to the larger ribosomal RNA gene are also reported.

Key Words:

• ITS1
• ITS2
• 5.8s rDNA
• Pinus pinea
• ribosomal DNA