Cloning and sequencing of the dnaK locus in Streptomyces coelicolor A3 (2)

Abstract

The dnaK operon of Streptomyces coelicolor A3(2) was cloned by the DNA-probing method using synthetic oligonucleotides designed on the basis of two of the most conserved regions in 30 different DnaK proteins (HSP'IO). The isolated insert—a BamHl 5.6–kb fragment—was sequenced and shown to contain three open-reading frames organized in an operon and coding for proteins analogous to DnaK, GrpE and DnaJ, successively.

Key Words:

• DnaJ
• chaperones
• grpE
• heat-shock proteins