Nucleotide and Predicted Peptide Sequence of Feline Interleukin-12 (IL-12)

Daniela Fehr Clinical Laboratory, Dept. of Internal Veterinary Medicine, University of Zurich, SwitzerlandCorrespondence[email protected]

Gregg A. Dean Dept. of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California, USA; Department of Microbiology, Pathology and Parasitology, College of Veterinary Medicine, North Carolina State University, Raleigh, USA

Jon Huder Clinical Laboratory, Dept. of Internal Veterinary Medicine, University of Zurich, Switzerland

Zhanyun Fan Dept. of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California, USA

Silke Huettner Clinical Laboratory, Dept. of Internal Veterinary Medicine, University of Zurich, Switzerland

Joanne W. Higgins Dept. of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California, USA

Neils C. Pedersen Dept. of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California, USA

Hans Lutz Clinical Laboratory, Dept. of Internal Veterinary Medicine, University of Zurich, Switzerland

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Abstract

Feline Interleukin-12 (IL-12) is a heterodimeric glycoprotein consisting of two disulfide linked subunits of about 40kD (p40) and 35kD (p35). It is a pleiotropic cytokine mediating biological activities on T- and NK-cells. One important function is the induction of a Thl immune response. Here we report the cloning and sequencing of feline IL-12, the expression of the p40-protein in E. coli and production of monoclonal antibodies. At the nucleotide level, feline IL-12 shows between 87-90%, on the amino acid level between 82-87% identity to the bovine and human IL-12, respectively.

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Nucleotide and Predicted Peptide Sequence of Feline Interleukin-12 (IL-12)

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Nucleotide and Predicted Peptide Sequence of Feline Interleukin-12 (IL-12)

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