Isolation and Characterization of the DNAKJ Operon from Actinobacillus actinomycetemcomitans

Akihiro Yoshida Department of Oral and Maxillofacial Surgery, Kyushu University Faculty of Dentistry, Maidashi, Higashi-ku, Fukuoka, 812-82, Japan

Yoshio Nakano Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Maidashi, Higashi-ku, Fukuoka, 812-82, JapanCorrespondence[email protected]

Yoshihisa Yamashita Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Maidashi, Higashi-ku, Fukuoka, 812-82, Japan

Hao Yu Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Maidashi, Higashi-ku, Fukuoka, 812-82, Japan

Masamichi Ohishi Department of Oral and Maxillofacial Surgery, Kyushu University Faculty of Dentistry, Maidashi, Higashi-ku, Fukuoka, 812-82, Japan

Toshihiko Koga Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Maidashi, Higashi-ku, Fukuoka, 812-82, Japan

Abstract

The dnaKJ operon of Actinobacillus actinomycetemcomitans Y4 was cloned by the DNA-probing method using synthetic oligonucleotides designed on the basis of two conserved regions in DnaK/hsp70 proteins and sequenced by inverse PCR. The sequenced region was shown to contain two open reading frames coding for proteins analogous to DnaK and DnaJ.

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Isolation and Characterization of the DNAKJ Operon from Actinobacillus actinomycetemcomitans

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Isolation and Characterization of the DNAKJ Operon from Actinobacillus actinomycetemcomitans

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