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Original Article

The Introns of the Canine Rod Opsin Gene Show Higher Sequence Homology to the Human than to the Rodent Introns

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Pages 99-104 | Received 17 Apr 1997, Published online: 11 Jul 2009
 

Abstract

Using genomic DNA from late-onset retinal degenerate and wild type Labrador Retrievers as templates and canine exon-specific oligonucleotides as primers in polymerase chain reaction, all four introns of opsin were cloned and sequenced. Dot-matrix comparisons were made for human, murine and canine introns. Selected sequences containing either intronic or coding sequences were aligned and used for phylogenetic relationship analysis.

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