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DNA Sequence Volume 9, 1998 - Issue 5-6
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Original Article

Sequencing Analysis of Forty-Eight Human Image cDNA Clones Similar to Drosophila Mutant Protein

Sara Volorio Telethon Institute of Genetics and Medicine (TIGEM), San Raffaede Biomedical Science Park, Via Olgettina 58, 20132, Milano, Italy
,
Gyorgy Simon Telethon Institute of Genetics and Medicine (TIGEM), San Raffaede Biomedical Science Park, Via Olgettina 58, 20132, Milano, Italy
,
Monica Repetto Telethon Institute of Genetics and Medicine (TIGEM), San Raffaede Biomedical Science Park, Via Olgettina 58, 20132, Milano, Italy
,
Maria Cucciardi Telethon Institute of Genetics and Medicine (TIGEM), San Raffaede Biomedical Science Park, Via Olgettina 58, 20132, Milano, Italy
,
Sandro Banfi Telethon Institute of Genetics and Medicine (TIGEM), San Raffaede Biomedical Science Park, Via Olgettina 58, 20132, Milano, Italy
,
Giuseppe Borsani Telethon Institute of Genetics and Medicine (TIGEM), San Raffaede Biomedical Science Park, Via Olgettina 58, 20132, Milano, Italy
,
Andrea Ballabio Telethon Institute of Genetics and Medicine (TIGEM), San Raffaede Biomedical Science Park, Via Olgettina 58, 20132, Milano, Italy
&
Massimo Zollo Telethon Institute of Genetics and Medicine (TIGEM), San Raffaede Biomedical Science Park, Via Olgettina 58, 20132, Milano, ItalyCorrespondence[email protected]
 show all
Pages 307-315 | Received 08 May 1998, Published online: 11 Jul 2009
 
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Abstract

We have sequenced 48 human IMAGE cDNA clones selected from the public EST database (dbEST) for their significant homology to Drosophila mutant genes. A dynamically updated analysis report was produced by BlastX and BlastN analysis searches in the latest databases available. This analysis led us to estimate the grade of similarity with homologous genes isolated in other species. Bottlenecks were detected in the sequencing process and here we have presented our problem-solving approach. We think the value of this full-length sequencing project is an enrichment of the sequence database information that is currently available to the human genome community.

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