Nucleotide Sequence of the GLABROUS1 Gene of Arabidopsis thaliana Ecotype Columbia

Abstract

The GLABROUS1 (GLl)gene from Arabidopsis thaliana ecotype Columbia was isolated and sequenced. Comparison of the nucleotide sequence with that of the gl1-2allele revealed that two sites were changed in gl1-2. One was a 14-bp deletion in exon 3, which seemed most likely to be the cause of the mutated phenotype. The other was a change from TC to CT in the 5′ untranslated region. Since the 5′ upstream sequence required for the GL1 gene expression has not been clearly specified, one cannot rule out the possibility that this change may contribute to the phenotype of gl1-2. A comparison was also made with the GL1 gene of ecotype Wassilewskija and revealed some lack of sequence conservation. The changes observed may have little influence on the function or the expression of the GL1 gene product.

Key Words:

• GL1
• Arabidopsis thaliana ecotype Columbia